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You are here:Home » Antibodies » Abs to Ubiquitin » Anti -SENP1 (Sentrin, SUMO-specific protease 1)

Anti -SENP1 (Sentrin, SUMO-specific protease 1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
The mammalian SUMO-specific protease (SENP1) is active against sentrin, but not ubiquitin or NEDD8-modified proteins in vivo (1). SENP1 provides a unique tool to study the role of sentrinization in the biological function of Pml and in the pathogenesis of acute promyelocytic leukemia (1). SENP1 localizes to the nucleus mainly in discrete subdomains, a subset of which co-localized with Pml nuclear bodies (NBs) (2). Both The herpes simplex virus 1 (HSV-1) immediate early regulatory protein (ICP0) and SENP1 protease promote the loss of SUMO-1 from the nucleus (2). SENP1 recruitment may be an important (though not sufficient) role in the modulation of SUMO-1 and NBs by ICP0 (2). SENP1 itself may be a target for SUMO-1 modification occurring at a nonconsensus site. SENP1 localization is influenced by expression and localization of SUMO-1-conjugated target proteins within the cell (3).
Catalog #S0725
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 1-3ug/ml
Immunoprecipitation: 7ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~0.25mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide derived from the mid region of the human SENP1 protein.
SpecificityReacts with the human SENP1 (~73kD). Reactivity has been confirmed with FLAG-HA-tagged-SENP1 transfected Hep2 cell lysates and human HL-60 acute myeloid leukemia cell lysates.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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