Anti -SENP3 (Sentrin-specific Protease 3, Sentrin/SUMO-specific Protease SENP3, SMT3IP1,
SUMO-1-specific Protease 3, SSP3, SUSP3)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Purified |
B IP IF |
|
| SENP3 is a member of the SENP (sentrin/SUMO-specific protease) family. The SUMO protease localizes to the nucleolus and catalyzes the release of SUMO2 and SUMO3 monomers from sumoylated substrates (1,2). SENP3 has been reported responsible for the removal of SUMO2/3 from many important target proteins, and regulates their function and stability. Desumoylation of MEF2D (removal of SUMO2/3) leads to an increase of MEF2D transcriptional activation (3). SENP3 enhances the binding of HIF-1a to p300 by deconjugation of SUMO2/3 from p300, leading to the upregulation of HIF-1a transcriptional activity and angiogenesis (4). SENP3 localizes to nucleolus through its binding to the nucleolar protein nucleophosmin | | | (NPM1) (5), and its deconjugation activity towards NPM1 is required for rRNA processing during ribosomal biogenesis (6). Under mild oxidative stress, SENP3 colocalizes with PML, and desumoylates and inhibits the function of PML to promote cell proliferation (7). SENP3 levels are tightly controlled in cells; NPM1, Arf, CHIP, and HSP90 have been shown to regulate the stability of SENP3, either by direct or indirect interaction (8,9). | | | Catalog # | S0742-06 | | Applications | Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested. | | Recommended Dilution | Immunofluorescence (IF-IC): 1:400 | | Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. | | Immunoprecipitation: 1:100 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 11C1068 | | Host | Rabbit | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM sodium chloride, 100ug/ml BSA, less than 0.02% sodium azide, 50% glycerol. | | Purity | Purified | | Immunogen | Synthetic peptide corresponding to residues surrounding Phe48 of human SENP3. | | Specificity | Recognizes endogeneous levels of total SENP3. Species Crossreactivity: monkey, mouse, rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | SUMO1/sentrin/SMT3 Specific Peptidase 3, DKFZp762A152, DKFZp586K0919, EC=3.4.22.- |
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