Small ubiquitin-related modifier proteins (SUMO-1, SUMO-2 and SUMO-3) are members of the ubiquitin-like protein family. SUMO-3 regulates Amyloid beta generation and may be critical in the onset or progression of Alzheimer’s disease. SUMO-2/3 forms poly-(SUMO) chains, which is conjugated to topoisomerase II and APP and regulates chromosomal segregation, progression of Alzheimer’s disease and cellular responses to environmental stress. SUMO-1, 2, and 3 proteins localize to the nuclear membrane, nuclear bodies, and cytoplasm, respectively.
Applications
Suitable for use in Western Blot. Other applications have not been tested.
Recommended Dilution
Western Blot: 1-3ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide derived from the C-terminal region of the human SUMO-3 protein
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Reacts with the human SUMO-3 protein also known as SMT3A, SMT3h1, or Sentrin-3. On Western blots, it identifies a single band at ~18kD. Several bands of unknown origin may be observed at ~25kD, ~35kD, and above. Reactivity has been confirmed with SK-OV3 human ovary adenocarcinoma or Jurkat human T lymphocyte Leukemia cell lysates.