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Specifications

Clone Host Grade Applications Monoclonal Mouse Ascites E B Gel

Protein phosphorylation and dephosphorylation reactions is a key postranslational event in the modification of protein functions. Protein phosphorylation occurs at tyrosine, serine, or threonine (p-tyr, p-ser, or p-thr) residues. Many different mitogenic systems, such as the EGF, PDGF, and insulin receptor systems contain tyr/ser/thr kinase domains which autophosphorylate specific tyr, ser or thr residues upon binding of their ligands. T cell antigen receptor complex or the receptors for some hemopoietic growth factors may stimulate associated kinases, and cells transformed by viral oncogenes contain elevated levels of phosphorylated proteins. An understanding of transformation by oncogenes and mitogenic processes of growth factors requires a delineation of all potential partners involved in phosphorylation cascade. The availability of antibodies specific for p-tyr, p-thr or p-thr residues has greatly advanced the studies on role of phosphorylated proteins.
Catalog #	S1000-75B
Applications	Suitable for use in ELISA, Dot Blot and Western Blot. Other applications not tested.
Recommended Dilution	ELISA: 1:1000-1:5000
	Western Blot: 1:500-1:2000
	Dot Blot: 1:500- 1:2000.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	11C149
Host	Mouse
Concentration	Not determined
Form	Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Purity	Ascites
Immunogen	O-Phospho-L-serine (KLH).
Specificity	Recognizes phosphorylated serine both as free amino acid or when conjugated to carriers such as BSA or KLH. Does not react with nonphosphorylated serine, phosphorylated tyrosine or threonine, AMP, or ATP.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.