Anti -SERPINB9 (Serine/Cysteine Proteinase Inhibitor, Clade B, Member 9, Serpin B9, PI9,
Cytoplasmic Antiproteinase 3, CAP-3, CAP3)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B IH FC |
|
| PI-9 (proteinase inhibitor 9), also known as SerpinB9, is a 42kD intracellular nucleocytoplasmic serpin expressed in cytotoxic lymphocytes (CTLs), natural killer (NK) cells, monocyte-derived dendritic cells (DCs), and to a lesser extent in B cells and myeloid cells. | | | Catalog # | S1000-89A | | Granzyme B (grB) is a serine protease highly expressed by CTLs and NK cells, which is endocytosed by virus-infected and malignant target cells. The subsequent release of grB from the endocytic vesicles into the cytoplasm of the target cells, triggers grB-mediated apoptosis, through cleavage of various cytoplasmic or nuclear proteins. PI-9, up-regulated in response to grB production and degranulation, has been identified as a potent inhibitor of Granzyme B-mediated apoptosis, providing a vital self-protection mechanism against the premature apoptosis of CTLs and NK cells by grB, which may escape into the cytoplasm of the effector cells themselves. | | Applications | Suitable for use in Flow Cytometry, Immunohistochemistry (paraffin), and Western Blot. Other applications not tested. | | Recommended Dilution | Flow Cytometry: 1:10-1:100 | | Immunohistochemistry (paraffin): 1:50 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 10F288 | | Host | Mouse | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, 0.05% sodium azide. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Recombinant PI-9 produced in P. pastoris. | | Specificity | Recognizes human PI-9 at ~42kD under reducing conditions. Does not react with porcine or mouse PI-9. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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