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You are here:Home » Antibodies » Abs to Protease Inhibitors » Anti -SERPINB9 (Serine/Cysteine Proteinase Inhibitor, Clade B, Member 9, Serpin B9, PI9, Cytoplasmic Antiproteinase 3, CAP-3, CAP3)

Anti -SERPINB9 (Serine/Cysteine Proteinase Inhibitor, Clade B, Member 9, Serpin B9, PI9,
Cytoplasmic Antiproteinase 3, CAP-3, CAP3)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH FC
PI-9 (proteinase inhibitor 9), also known as SerpinB9, is a 42kD intracellular nucleocytoplasmic serpin expressed in cytotoxic lymphocytes (CTLs), natural killer (NK) cells, monocyte-derived dendritic cells (DCs), and to a lesser extent in B cells and myeloid cells.
Catalog #S1000-89A
Granzyme B (grB) is a serine protease highly expressed by CTLs and NK cells, which is endocytosed by virus-infected and malignant target cells. The subsequent release of grB from the endocytic vesicles into the cytoplasm of the target cells, triggers grB-mediated apoptosis, through cleavage of various cytoplasmic or nuclear proteins. PI-9, up-regulated in response to grB production and degranulation, has been identified as a potent inhibitor of Granzyme B-mediated apoptosis, providing a vital self-protection mechanism against the premature apoptosis of CTLs and NK cells by grB, which may escape into the cytoplasm of the effector cells themselves.
ApplicationsSuitable for use in Flow Cytometry, Immunohistochemistry (paraffin), and Western Blot. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:10-1:100
Immunohistochemistry (paraffin): 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No10F288
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant PI-9 produced in P. pastoris.
SpecificityRecognizes human PI-9 at ~42kD under reducing conditions. Does not react with porcine or mouse PI-9.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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