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You are here:Home » Antibodies » Antibodies-Serum Proteins » Anti -Serotonin (5-Hydroxytryptamine, 5HT)

Anti -Serotonin (5-Hydroxytryptamine, 5HT)


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Clone Host Grade Applications
Polyclonal Rabbit Serum IH
Catalog #S1001-10C
Applications Suitable for use in Immunohistochemistry.
Recommended DilutionImmunohistochemistry: 1:500-1:2000. Paraffin, Frozen. Immunostaining is abolished by pre-incubation of the diluted antiserum with 100ug/ml 5-HT.
Does not require protein digestion pre-treatment of paraffin sections.Does not require antigen retrieval using heat pre-treatment prior to staining of paraffin sections. Gluterablehyde (0.5-4%) should be included in the fixative. Metabisulfite and borohydride reduction steps are required.
Optimal working dilutions to be determined by researcher.
Recommended Secondary Reagents Sheep anti Rabbit IgG: HRP
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
ConcentrationNot determined
FormSerum. Supplied as a liquid with 0.1% sodium azide.
PurityNeat serum
ImmunogenSerotonin coupled to BSA.
SpecificityRecognizes serotonin (5-HT). Species Crossreactivity: Human, rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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