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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B IH

The serotonin receptors, also known as 5-hydroxytryptamine receptors or 5-HT receptors, are a group of G protein-coupled receptors (GPCRs) and ligand-gated ion channels (LGICs) found in the central and peripheral nervous systems. They mediate both excitatory and inhibitory neurotransmission. The serotonin receptors are activated by the neurotransmitter serotonin, which acts as their natural ligand.
Catalog #	S1001-15A
Applications	Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions	Western Blot: Using rat brain extracts of cortex, hypothalamus, midbrain and hindbrain, the antibody specifically labels a single band. Immunolabeling is abolished by pre-adsorption with synthetic rat 5HT transporter (aa579-599).
	Immunohistochemistry (indirect IF): 1:200-1:400
	Immunohistochemistry (biotin/streptavidin HRP technique): 1:200-1:600 in rat raphe nuclei, hypothalamus, cortex and spinal cord.
	Optimal dilution determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Rat
Concentration	~0.1mg/ml
Form	Supplied as a liquid, 1% BSA.
Purity	Purified by affinity chromatography.
Immunogen	Synthetic peptide corresponding to aa294-312 of the rat 5-HT1A receptor coupled to bovine thyroglobulin with glutaraldehyde.
Specificity	Recognizes rat Serotonin Receptor 1A. Species Crossreactivity: human and mouse.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.