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Specifications

Clone Host Grade Applications Monoclonal Mouse Highly Purified B IH

Serotonin Receptor 1A, also known as 5-hydroxytryptamine receptor 1A, is a member of the G protein-coupled receptor family. Serotonin Receptor 1A has been found to be expressed highly in the septum and hippocampus. Serotonin Receptor 1A seems to have an inhibitory effect on Ca2+ channels, via N-channels and L-channels. The receptor actuates voltage-sensitive slowing using the N-channels and actuates steady state inhibition using the L-channels.
Catalog #	S1001-15C
Applications	Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions
	Western Blot: 0.5ug/ml detects Serotonin Receptor 1A on 10ug of rat brain tissue lysate. Band is observed at ~40kD. Calculated MW is 46kD.
	Immunohistochemistry: 1:400 detects Serotonin Receptor 1A in normal human brain tissue.
	Optimal dilutions to be determined by the researcher.
Positive Control	Rat brain tissue lysate
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgM,k
Clone No	11C254
Host	Mouse
Source	Rat
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, 0.05% sodium azide.
Purity	Purified by ion exchange chromatography.
Immunogen	Linear peptide corresponding to rat Serotonin Receptor 1A, conjugated to KLH ( NP_036717).
Specificity	Recognizes rat Seratonin receptor 1A. Species Crossreactivity: human.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.