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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B IH

Catalog #	S1001-23D
Applications	Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
	Recommended Dilutions:
	Western Blot: >1:100
	Immunohistochemistry: 1:500-1:1000 in PBS.
	Bn/SA-HRP Technique:
	Perfusion Fixation: Fixative-4% paraformaldehyde in 0.1M PBS, pH 7.4; 500ml over ~20 minutes.
	Post Fixation: 4% paraformaldehyde in 0.1M PBS, pH 7.4; 500ml over ~20 minutes.
	Post Fixation: 1.5 hour at 4°C. in 4% paraformaldehyde in 0.1M PBS, pH 7.4.
	Note: Paraformaldehyde is a necessary component in fixation of serotonin. If needed, low levels of glutaraldehyde (0.1-0.3%) may be used in conjunction with paraformaldehyde.
	Incubation on Tissue: 16-24 hours at 4ºC.
	Optimal dilutions to be determined by the researcher.
Positive Control
	Rat cortex and hippocampus
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Rat
Concentration	Not Determined
Form	Supplied as a lyophilized powder. Reconstitute with 100ul sterile ddH2O. Dilute further with PBS or Tris buffer.
Purity	Purified by affinity chromatography.
Immunogen	Synthetic peptide corresponding to aa 8-23 of the rat 5-HT7 receptor
Specificity	Recognizes rat Serotonin Receptor 7.
Species Crossreactivity	Mouse and human
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.