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You are here:Home » Antibodies » Abs to Serotonin Receptors » Anti -Serotonin Receptor 7 (5-HT7)

Anti -Serotonin Receptor 7 (5-HT7)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
Catalog #S1001-23D
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions:
Western Blot: 1:100
Immunohistochemistry (Bn-SA/HRP Technique): 1:100-1:300 in PBS.
Immunohistochemistry (Bn/Av-HRP Technique): 1:500-1:1000 in PBS.
Optimal dilutions to be determined by the researcher.
ImmunohistochemistryPerfusion Fixation: Fixative-4% paraformaldehyde in 0.1M PBS, pH 7.4; 500ml over ~20 minutes.
Post Fixation: 4% paraformaldehyde in 0.1M PBS, pH 7.4; 500ml over ~20min at RT or 1.5hr at 4°C. Note: Paraformaldehyde is a necessary component in fixation of serotonin. If needed, low levels of glutaraldehyde (0.1-0.3%) may be used in conjunction with paraformaldehyde.
Sections: 50um vibratome
Adsorption control: Synthetic peptide corresponding to aa8-23 of the rat 5-HT7 receptor
Incubation on Tissue: 16-24 hours at 4ºC.
Positive Control
Rat cortex and hippocampus tissues
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
ConcentrationNot Determined
FormSupplied as a lyophilized powder. Reconstitute with 100ul sterile ddH2O. Dilute further with PBS or Tris buffer.
PurityPurified by affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa 8-23 of the rat 5-HT7 receptor
SpecificityRecognizes rat Serotonin Receptor 7.
Species CrossreactivityMouse and human
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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