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You are here:Home » Antibodies » Abs to Amyloid » Anti -Serum Amyloid A4, Constitutive (SAA4, CSAA)

Anti -Serum Amyloid A4, Constitutive (SAA4, CSAA)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
Serum amyloid A4 (SAA4) is a constitutively expressed SAA protein that is also known as C-SAA. It is an apolipoprotein that constitutes more than 90% of SAA on HDL during homeostasis and may be involved in lipid transfer between lipoprotein classes. Mature human SAA4 contains 112 amino acid (aa) residues. It is primarily produced in hepatocytes and secreted plasma. In solution, SAA4 is reported to form stable dimers and trimers. Human and mouse SAA4 share 57% aa sequence identity.
Catalog #S1002-60A
ApplicationsSuitable for use in Direct ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsDirect ELISA: 0.5-1.0ug/ml; The detection limit for recombinant human SAA4 is ~0.1ng/well.
Western Blot: 0.1-0.2ug/ml; The detection limit for recombinant human SAA4 is ~0.5ng/lane under non-reducing and reducing conditions.
Immunohistochemistry: 2-15ug/ml will detect SAA4 in cells and tissues.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.2mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
PurityPurified by affinity chromatography.
ImmunogenRecombinant protein corresponding to aa19-130 of human SAA4 (P35542) expressed in E. coli.
SpecificityRecognizes human SAA4. Species Crossreactivity: recombinant mouse SAA4 (~20%). Shows <5% crossreactivity with recombinant human SAA1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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