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You are here:Home » Antibodies » Abs to Enzymes, Methyltransferase » Anti -SETDB1 (SET Domain Bifurcated 1, ERG-associated Protein with a SET Domain, ESET, Histone H3 K9 methyltransferase 4, Histone Lysine N-methyltransferase H3 Lysine 9 Specific 4, H3 K9 HMTase 4, H3-K9-HMTase 4, KG1T, KIAA00067) (Azide Free)

Anti -SETDB1 (SET Domain Bifurcated 1, ERG-associated Protein with a SET Domain, ESET,
Histone H3 K9 methyltransferase 4, Histone Lysine N-methyltransferase H3 Lysine 9 Specific
4, H3 K9 HMTase 4, H3-K9-HMTase 4, KG1T, KIAA00067) (Azide Free)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IF
Methylation of histone tails plays an important role in chromatin structure and function. Previously, we reported that ESET/SETDB1 is a histone methyltransferase (HMTase). It has been shown that SETDB1 tightly associates with the human homolog of mAM, a murine ATFa-associated factor. Although recombinant ESET can methylate lysine 9 of histone H3 (H3-K9), its activity is severely compromised when compared to that of the ESET/mAM complex. mAM stimulates ESET enzymatic activity by increasing the Vmax and decreasing the Km. Importantly, mAM facilitates the ESET-dependent conversion of dimethyl H3-K9 to the trimethyl state both in vitro and in vivo. Chromatin-based transcription and ChIP analyses demonstrate that mAM enhances ESET-mediated transcriptional repression in a SAM-dependent manner, and this repression correlates with H3-K9 trimethylation at the promoter. Thus, our studies establish that promoter H3-K9 trimethylation is the cause of transcriptional repression and that mAM/hAM facilitates conversion of H3-K9 dimethyl to trimethyl by ESET/SETDB1.
Catalog #S1010-68B
ApplicationsSuitable for use in ELISA, Immunofluorescence and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:500-1:1000
ELISA: 1ug/ml-3ng/ml
Optimal dilutions to be determined by the researcher.
HybridomaSp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No10B2474
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2. No preservative added (Azide free).
PurityPurified by Protein A affinity chromatography.
ImmunogenRecombinant protein corresponding to aa2-101 of human SETDB1.
SpecificityRecognizes human SETDB1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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