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You are here:Home » Antibodies » Abs to Glucose Transporters » Anti -SGLT-2 (Sodium Glucose Transporter 2)

Anti -SGLT-2 (Sodium Glucose Transporter 2)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
The kidneys play a major role in the regulation of glucose levels. Kidneys filter approx. 180g of glucose per day from the blood, and this is mostly reabsorbed back into the blood in the proximal tubules. Typically, glucose is first absorbed within epithelium by a specific transporter protein, Sodium glucose co- transporters (SGLT), in the brush-border membrane and then it is transported out of the cell across the basolateral membranes by a facilitated sugar transporter (GLUTs). At least 3 members of SGLTs (SGLT1-3) have been cloned and characterized from various species. Individual members of this family have identical predicted secondary structures with up to 14 transmembrane domains. SGLT1-3 genes code for protein of ~659-672aa (calculated size of ~75kD). Both N and C-termini are predicted to be extracellualr. There is ~60-70% homology between SGLT1-3. SGLTs transport a-methyl-D-glucoside (a-MDG), a non-metabolized model substrate, in Na-dependent manner. SGLT1 does not discriminate among a-MDG, glucose, and galactose. SGLT2/3 do not transport D-galactose efficiently.
SGLT2/SLC5A2 (rat/mouse 670 aa; human 672 aa, chromosome 16p11.2) is the low affinity, high capacity Na+-glucose transporter located in the S1 segments of proximal tubules. It is ~60% identical with SGLT1. SGLT2 mediates saturable Na-dependent and phlorizin-sensitive glucose transport. Defects in SGLT2 may be associated with renal glycosuria.
Catalog #S1010-87B
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1-10ug/ml using ECL.
ELISA: 0.5-1ug/ml.
Optimal dilutions to be determined by the researcher.
Control Peptide: S1010-87A
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% BSA, 40% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenA 16aa synthesic, peptide from the N-terminal, cytoplasmic domain 1 of Rat SGLT-2 (KLH).
SpecificityRecognizes rat SGLT-2. No significant sequence homology exists with other SGLTs. Species sequence homology: mouse 86% .
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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