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You are here:Home » Antibodies » Abs to Glucose Transporters » Anti -SGLT-2 (Sodium Glucose Transporter 2)

Anti -SGLT-2 (Sodium Glucose Transporter 2)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B
Catalog #S1010-87C
ApplicationsSuitable for use in ELISA, Western Blotting. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:5000 (ECL). A band of 95-110kD has been detected in brush border membrane (2).
ELISA: 1:10,000-50,000 . Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Control Peptide S1010-87A
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
ConcentrationAs reported
FormSupplied as a liquid, neat serum, 40% glycerol.
PuritySerum
ImmunogenA 16 AA peptide sequence from the N-terminal, cytoplasmic domain 1 of Rat SGLT-2 (1) was synthesized, coupled to KLH
SpecificityThe rat peptide sequence is ~67% conserved in mouse SGLT2. No significant sequence homology exists with other SGLTs. For human SGLT2, we recommend the use antibody that is made to the human SGLT-2 peptide. Control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10 ug control peptide per 1 ug of aff pure IgG or 1 ul antiserum) to confirm antibody specificity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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