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You are here:Home » Antibodies » Abs to Adapter Proteins » Anti -Shc, p66 (SHC1, Src Homology and Collagen, Src Homology 2, FLJ26504, p52SHC, p66SHC, SH2 Domain Protein C1, SHC, SHCA, SHC Transforming Protein 1)

Anti -Shc, p66 (SHC1, Src Homology and Collagen, Src Homology 2, FLJ26504, p52SHC, p66SHC,
SH2 Domain Protein C1, SHC, SHCA, SHC Transforming Protein 1)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
The human Src homology and collagen (SHC) gene encodes for three proteins (p46, p52, p66). All three SHCs are known to be signaling adaptors that couple activated growth factor receptors to their signaling pathways. p52 and p46 are translated from the same mRNA using two different initiation codons while p66 is generated form a distinct mRNA. p52 and p46 are expressed in every cell type while p66 expression varies from cell type to cell type. Upon stimulation by tyrosine kinase receptors and cytoplasmic TKs, all three SHCs become phosphorylated. Once phosphorylated, p52, p46 and p66 recruit and form a stable complex with GRB2/SOS. p52 and p46 activate the Ras and MAP kinase pathway, while p66 is involved in a signal transduction pathway that regulates the cellular response to oxidative stress and life span.
Catalog #S1010-90
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested.
Recommended DilutionsWestern Blot: 0.5-2ug/ml detects Shc isoforms in RIPA lysates from human A431 carcinoma cells, and previous lots detected Shc in mouse 3T3 RIPA cell lysate. A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with S1010-90. Proteins were visualized using an IgG
(HRP) goat anti-rabbit secondary antibody and a chemiluminescence detection system.
Immunoprecipitation: 4ug immunoprecipitates SHC isoforms from a human A431 RIPA cell lysate.
Optimal dilutions to be determined by the researcher.
Positive Control IncludedA0001-50: Non-stimulated A431 cell lysate. Add 2.5ul of 2 mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Use 20ug of reduced lysate per lane for minigels.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.
PurityPurified by Protein A affinity chromatography.
ImmunogenRecombinant GST fusion protein corresponding to aa366-473 of human SHC-SH2.
SpecificityRecognizes the human 46kD, 52kD and 66kD SHC proteins. Species Crossreactivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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