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You are here:Home » Antibodies » Abs to Adapter Proteins » Anti -Shc, phosphorylated (Tyr239, Tyr240) (SHC1, Src homology and collagen, Src homology 2, FLJ26504, p52SHC, p66SHC, SH2 domain protein C1, SHC, SHCA, SHC transforming protein 1)

Anti -Shc, phosphorylated (Tyr239, Tyr240) (SHC1, Src homology and collagen, Src homology
2, FLJ26504, p52SHC, p66SHC, SH2 domain protein C1, SHC, SHCA, SHC transforming protein 1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Shc is a signaling and transforming protein that binds Src homology 2 and 3 (SH2 and SH3) domains. Shc mediates the signaling from many types of receptors, such as those for growth factors, antigens, cytokines, integrins and G-protein coupled receptors, to their downstream proteins. This downstream signaling occurs via binding to Grb2, an adaptor protein for the Ras exchange factor Sos, leading to activation of the Ras/Raf/MAPK pathway. There are three isoforms of Shc (p46, p52 and p66), where p46 and p52 are the most widely expressed. Phosphorylation of Shc at tyrosines 239, 240 and 317 potentiates its function as a regulatory adaptor protein.
Catalog #S1010-90B1
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.1-1ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlA431 cells +/- EGF
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic phosphopeptide corresponding to human Shc that contains tyrosines 239 and 240. Species sequence homology: mouse.
SpecificityRecognizes human Shc when phosphorylated when Tyr239 at Tyr240. Mouse Shc has not been tested. Cross-reacts with insulin receptor in CHO cells over-expressing insulin receptor.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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