Anti -Shc, p66, NT (SHC1, Src Homology and Collagen, Src Homology 2, FLJ26504, p52SHC,
p66SHC, SH2 Domain Protein C1, SHC, SHCA, SHC Transforming Protein 1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Highly Purified |
E B |
|
| The human Src homology and collagen (SHC) gene encodes for three proteins (p46, p52, p66). All three SHCs are known to be signaling adaptors that couple activated growth factor receptors to their signaling pathways. p52 and p46 are translated from the same mRNA using two different initiation codons while p66 is generated from a distinct mRNA. p52 and p46 are expressed in every cell type while p66 expression varies from cell type to cell type. Upon stimulation by tyrosine kinase receptors and cytoplasmic TKs, all three SHCs become phosphorylated. Once phosphorylated, p52, p46 and p66 recruit and form a stable complex with GRB2/SOS. p52 and p46 activate the Ras and MAP kinase pathway, while p66 is involved in a signal transduction pathway that regulates the cellular response to oxidative stress and life span. | | | Catalog # | S1010-90C2 | | Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. | | Recommended Dilutions | Western Blot: 0.5ug/ml for HRP/ECL detection. | | Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. | | Optimal dilutions to be determined by the researcher | | Positive Control Included | | | N1225-02: Neuro 2A Cell Lysate | | Add 100ul SDS-PAGE sample buffer and incubate at 90°C for 5 minutes. Aliquot and store at -20°C | | Western Blot: 20ul/lane (mini gel) for HRP/ECL detection. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 6D757 | | Host | Mouse | | Source | Human | | Concentration | ~0.1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, PEG, sucrose, 50% glycerol. | | Purity | Purified by subsequent thiophillic adsorption and size exclusion chromatography. | | Immunogen | Synthetic peptide corresponding to a portion of N-terminal p66Shc. | | Specificity | Recognizes the N-terminus of human p66Shc at ~66kD. Species Crossreactivity: mouse, rat and canine. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Anti -Shc, p66, NT (SHC1, Src Homology and Collagen, Src Homology 2, FLJ26504, p52SHC, p66SHC, SH2 Domain Protein C1, SHC, SHCA, SHC Transforming Protein 1) |
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