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You are here:Home » Antibodies » Abs to Adapter Proteins » Anti -Shc, phosphorylated (Tyr317) (SHC1, Src homology and collagen, Src homology 2, FLJ26504, p52SHC, p66SHC, SH2 domain protein C1, SHC, SHCA, SHC transforming protein 1)

Anti -Shc, phosphorylated (Tyr317) (SHC1, Src homology and collagen, Src homology 2,
FLJ26504, p52SHC, p66SHC, SH2 domain protein C1, SHC, SHCA, SHC transforming protein 1)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B
The human Src homology and collagen (SHC) gene encodes for three proteins (p46, p52, p66). All three SHCs are known to be signaling adaptors that couple activated growth factor receptors to their signaling pathways. p52 and p46 are translated from the same mRNA using two different initiation codons while p66 is generated form a distinct mRNA. p52 and p46 are expressed in every cell type while p66 expression varies from cell type to cell type. Upon stimulation by tyrosine kinase receptors and cytoplasmic TKs, all three SHCs become phosphorylated. Once phosphorylated, p52, p46 and p66 recruit and form a stable complex with GRB2/SOS. p52 and p46 activate the Ras and MAP kinase pathway, while p66 is involved in a signal transduction pathway that regulates the cellular response to oxidative stress and life span.
Catalog #S1010-90D
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot Analysis: 0.5-2ug/ml detects phosphorylated SHC in whole cell lysates from HFF cells stimulated with 5ng/ml EGF for 10 minutes. Non-stimulated or EGF-stimulated HFF cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-SHC (Y317) S1010-90D ( 0.5ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodyI1904-77B: IgG1 (HRP) Pab GtxMo
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No3J19
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 0.09% sodium azide.
PurityPuri ed from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
ImmunogenSynthetic peptide corresponding to aa314-320 (DPSpYVNV) of human p52 SHC (KLH).
SpecificityRecognizes human phosphorylated SHC protein isoforms, Mr 46, 52 and 67kD. Species Crossreactivity: Mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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