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You are here:Home » Antibodies » Abs to Adapter Proteins » Anti -Shc, phosphorylated (Tyr427) (SHC-transforming Protein 1, SHC-transforming Protein 3, SHC-transforming Protein A, Src Homology 2 Domain-containing-transforming Protein C1, SH2 Domain Protein C1, SHC1, SHCA)

Anti -Shc, phosphorylated (Tyr427) (SHC-transforming Protein 1, SHC-transforming Protein
3, SHC-transforming Protein A, Src Homology 2 Domain-containing-transforming Protein C1,
SH2 Domain Protein C1, SHC1, SHCA)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
Shc possesses SH2 and PTB domains. It serves a scaffolding function in signaling for a variety of receptor tyrosine kinases. Shc exists in p46, p52 and p66 isoforms. These isoforms are produced by using alternative translation initiation sites or a differentially spliced message. In response to extracellular signals, the SH2 and PTB domains of Shc interact with the activated receptors, leading to phosphorylation of Shc on three different tyrosine residues: Tyr239, Tyr240 and Tyr317. Grb2/Sos binds to Shc phosphorylated at these sites, activating the Ras/Raf/MAPK pathway. Both Shc expression and its tyrosine phosphorylation play an essential and nonredundant role in thymic T cell development.
Catalog #S1010-90L
ApplicationsSuitable for use in Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot:1:500-1:1,000
Immunohistochemistry:1:50-1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic phosphopeptide corresponding to human Shc1 around the phosphorylation site of tyrosine 427 (P-S-YP-V-N).
SpecificityRecognizes human Shc1 when phosphorylated at (Tyr427). Species Cross-Reactivity: mouse, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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