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Anti -SH2 Domain Protein 2A (SH2D2A, Lck-associated Adapter Protein, Lad, Rlk/Itk-binding Protein, RIBP)
Anti -SH2 Domain Protein 2A (SH2D2A, Lck-associated Adapter Protein, Lad, Rlk/Itk-binding
Protein, RIBP)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Sheep |
Affinity Purified |
B |
|
| RIBP (Rlk/Itk-binding protein; also SH2D2A, LAD/Lck-associated adapter protein, MEKK2-binding protein and TSAd) is a 45-47kD cytoplasmic adaptor protein. It is found in B cells, thymocytes, NK cells and T cells, and appears to be necessary for TCR-initiated proliferation, plus IL-2 and IFN-gamma secretion. This is likely to be a function of its association with MEKK2 near the T cell receptor interaction site. Mouse RIBP is 374aa in length and contains one SH2 domain (aa116-207), plus a Pro-rich region (aa267-308) that contains an SH3-binding motif (aa267-273). Phosphorylation may occur on Ser237, Ser316 and Tyr325. There is one alternative splice form termed LAD that shows a deletion of aa254-261. Over aa299-374, mouse RIBP shares 79% and 63% aa identity with rat and the presumed human RIBP ortholog (termed TSAd/SH2D2A), respectively. | | | Catalog # | S1010-90P | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilutions | Western Blot: 1ug/ml Detects a band at ~45kD. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS, 0.02% sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Sheep | | Source | Mouse | | Concentration | Not determined | | Form | Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with PBS, 0.02% sodium azide. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Purified, E. coli-derived recombinant mouse RIBP (rmRIBP; aa299-374; Accession # Q9QXK9). | | Specificity | Recognizes mouse SH2D2A. Species Crossreactivity: rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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