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You are here:Home » Antibodies » Abs to Protein Kinase Substrates » Anti -SHC (SHC-transforming Protein 1, SH2 Domain Protein C1, SHC1, SHC-transforming Protein 3, SHC-transforming Protein A, SHCA, Src Homology 2 Domain-containing-transforming Protein C1, FLJ26504)

Anti -SHC (SHC-transforming Protein 1, SH2 Domain Protein C1, SHC1, SHC-transforming
Protein 3, SHC-transforming Protein A, SHCA, Src Homology 2 Domain-containing-transforming
Protein C1, FLJ26504)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
Shc1 is an adapter protein in the Ras-MAPkinase pathway that is involved in the regulation of cell growth and differentiation. The protein contains both PTB and SH2 domains and becomes phosphorylated on tyrosine in response to many different extracellular stimuli. human Shc1 adaptor protein is encoded by one gene but has three isoforms: p66SHC1, p52SHC1 and p46SHC1. Both p52SHC1 and p46SHC1 are associated with MAPK signaling and are highly conserved throughout evolution; p66SHC1 signaling is associated with apoptosis and is only seen in vertebrates. The SHC1 gene contains one known coding variation, which corresponding to Met225Val in the p46 isoform, Met300Val in the p52 isoform and to Met410Val in the p66 isoform. Recent studies confirms Shc1 an important candidate gene for longevity in humans. The protein is widely expressed including the neural stem cells.
Catalog #S1010-90V
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:500-1:1000
Immunohistochestry (paraffin): 1:50-1:100
Optimal dilutions to be determined by the researcher.
Positive Control293 cells
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic non-phosphopeptide corresponding to human Shc1 around the phosphorylation site of tyrosine 427 (P-S-YP-V-N).
SpecificityRecognizes human Shc1. Species crossreactivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesSHC (Src Homology 2 Domain Containing) Transforming Protein 1


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