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You are here:Home » Antibodies » Abs to Adapter Proteins » Anti -Shc (Y239), phosphorylated (SHC1, Src homology and collagen, Src homology 2, FLJ26504, p52SHC, p66SHC, SH2 domain protein C1, SHC, SHCA, SHC transforming protein 1)

Anti -Shc (Y239), phosphorylated (SHC1, Src homology and collagen, Src homology 2,
FLJ26504, p52SHC, p66SHC, SH2 domain protein C1, SHC, SHCA, SHC transforming protein 1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
The human Src homology and collagen (SHC) gene encodes for three proteins (p46, p52, p66). All three SHCs are known to be signaling adaptors that couple activated growth factor receptors to their signaling pathways. p52 and p46 are translated from the same mRNA using two different initiation codons while p66 is generated form a distinct mRNA. p52 and p46 are expressed in every cell type while p66 expression varies from cell type to cell type. Upon stimulation by tyrosine kinase receptors and cytoplasmic TKs, all three SHCs become phosphorylated. Once phosphorylated, p52, p46 and p66 recruit and form a stable complex with GRB2/SOS. p52 and p46 activate the Ras and MAP kinase pathway, while p66 is involved in a signal transduction pathway that regulates the cellular response to oxidative stress and life span.
Catalog #S1010-91
Applications Suitable for use in ELISA, Western Blot.
Recommended DilutionWestern Blot: 1:20001:5000 detects phosphorylated SHC in RIPA lysates from HFF cells stimulated with 50ng/ml EGF for 10 minutes.
ELISA Specificity for pY239 was determined by ELISA, which compared antibody binding to unphosphorylated, dually phosphorylated, and mono-phosphorylated peptides. HFF cell lysates stimulated with EGF or not stimulated were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho SHC (Y239), 1:2000 dilution. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
FormSupplied as a liquid in 50mM Tris, pH 7.4; 150mM sodium chloride, 5ug/ml BSA, 0.05% sodium azide, 30% glycerol.
PurityImmunoaffinity purified
ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids 236243 of human SHC (DHQ[pY][pY]NDF-C). The immunizing sequence is identical in mouse.
SpecificityRecognizes phosphorylated SHC protein (Grb2 binding site), Mr 46kD, 52kD and 67kD. May crossreact with other proteins containing Grb2 binding site. Species Crossreactivity: Human; predicted crossreactivity with mouse based on sequence homology.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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