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You are here:Home » Antibodies » Abs to Adapter Proteins » Anti -Shc (SHC1, Src homology and collagen, Src homology 2, FLJ26504, p52SHC, p66SHC, SH2 domain protein C1, SHC, SHCA, SHC transforming protein 1)

Anti -Shc (SHC1, Src homology and collagen, Src homology 2, FLJ26504, p52SHC, p66SHC, SH2
domain protein C1, SHC, SHCA, SHC transforming protein 1)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP FC
The human Src homology and collagen (SHC) gene encodes for three proteins (p46, p52, p66). All three SHCs are known to be signaling adaptors that couple activated growth factor receptors to their signaling pathways. p52 and p46 are translated from the same mRNA using two different initiation codons while p66 is generated form a distinct mRNA. p52 and p46 are expressed in every cell type while p66 expression varies from cell type to cell type (1-3). Upon stimulation by tyrosine kinase receptors and cytoplasmic TKs, all three SHCs become phosphorylated. Once phosphorylated, p52, p46 and p66 recruit and form a stable complex with GRB2/SOS. p52 and p46 activate the Ras and MAP kinase pathway, while p66 is involved in a signal transduction pathway that regulates the cellular response to oxidative stress and life span (4-5).
Catalog #S1010-91B
ApplicationsSuitable for use in Flow Cytometry, Western Blotting, Immunoprecipitation. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:80
Western Blotting:1:2,000 (66/52/46kD)
Immunoprecipitation: 1:50 1ug/mg of total cell protein (~10e7 cells)
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No6k364
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid.
PuritySupernatant
ImmunogenA synthetic peptide corresponding to residues in the SH2 domain of human SHC1.
SpecificityRecognizes human SHC1. Detects p46, p52 and p56.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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