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You are here:Home » Antibodies » Abs to Adapter Proteins » Anti -Shc, phosphorylated (Y317) (SHC1, Src homology and collagen, Src homology 2, FLJ26504, p52SHC, p66SHC, SH2 domain protein C1, SHC, SHCA, SHC transforming protein 1)

Anti -Shc, phosphorylated (Y317) (SHC1, Src homology and collagen, Src homology 2,
FLJ26504, p52SHC, p66SHC, SH2 domain protein C1, SHC, SHCA, SHC transforming protein 1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
The human Src homology and collagen (SHC) gene encodes for three proteins (p46, p52, p67). All three SHCs are known to be signaling adaptors that couple activated growth factor receptors to their signaling pathways. p52 and p46 are translated from the same mRNA using two different initiation codons while p67 is generated form a distinct mRNA. p52 and p46 are expressed in every cell type while p67 expression varies from cell type to cell type. Upon stimulation by tyrosine kinase receptors and cytoplasmic TKs, all three SHCs become phosphorylated. Once phosphorylated, p52, p46 and p67 recruit and form a stable complex with GRB2/SOS. p52 and p46 activate the Ras and MAP kinase pathway, while p67 is involved in a signal transduction pathway that regulates the cellular response to oxidative stress and life span.
Catalog #S1010-92
ApplicationsSuitable for use in Western Blot. Other applications have not been tested.
Recommended DilutionWestern Blot Analysis: A 1:250-1:1000 dilution detects phosphorylated SHC in RIPA lysates from HFF cells stimulated with 50ng/ml EGF for 10 minutes. Non-stimulated or EGF-stimulated HFF cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with S1010-92, 1:1000 dilution. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid in 0.2M Tris-glycine, 150mM sodium chloride, 5mg/ml BSA, 0.05% sodium azide, before the addition of glycerol to 30%.
PurityPurified by immunoaffinity chromatography
ImmunogenSynthetic peptide (FDDPS[pY]VN-C) corresponding to aa312-319 of human p52 SHC, containing a C-terminal cysteine (KLH). This motif is the Grb2 binding site.
SpecificityRecognizes phosphorylated human SHC protein isoforms, Mr 46kD, 52kD and 67kD. In long exposures, additional proteins of Mr 110 and 180 are detected in EGF-treated lysates. Species Crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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