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You are here:Home » Antibodies » Abs to Adapter Proteins » Anti -Shc, phosphorylated (Tyr239, Tyr240) (SHC1, Src homology and collagen, Src homology 2, FLJ26504, p52SHC, p66SHC, SH2 domain protein C1, SHC, SHCA, SHC transforming protein 1)

Anti -Shc, phosphorylated (Tyr239, Tyr240) (SHC1, Src homology and collagen, Src homology
2, FLJ26504, p52SHC, p66SHC, SH2 domain protein C1, SHC, SHCA, SHC transforming protein 1)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B IH
The human Src homology and collagen (SHC) gene encodes for three proteins (p46, p52, p66). All three SHCs are known to be signaling adaptors that couple activated growth factor receptors to their signaling pathways. p52 and p46 are translated from the same mRNA using two different initiation codons while p66 is generated form a distinct mRNA. p52 and p46 are expressed in every cell type while p66 expression varies from cell type to cell type. Upon stimulation by tyrosine kinase receptors and cytoplasmic TKs, all three SHCs become phosphorylated. Once phosphorylated, p52, p46 and p66 recruit and form a stable complex with GRB2/SOS. p52 and p46 activate the Ras and MAP kinase pathway, while p66 is involved in a signal transduction pathway that regulates the cellular response to oxidative stress and lifespan.
Catalog #S1010-92K
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsELISA: 0.05ug/ml
Western Blot: 0.5ug/ml for HRPO/ECL detection. Casein/Tween-20 based blocking and blot incubations buffers are recommended.
Positive Control IncludedH2030-03H1: HepG2 Cell Lysate, Vanadate Treated
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No6D761
HostMouse
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, PEG, sucrose
PurityPurified by thiophilic adsorption and size exclusion chromatography.
ImmunogenSynthetic peptide corresponding to aa236-243, DHQpYpYNDF of shc phosphorylated at Tyr239 and Tyr240, conjugated to KLH
SpecificityRecognizes shc phosphorylated at Tyr239/240. Does not crossreact with non-phosphorylated shc.
Species CrossreactivityHuman, mouse and canine
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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