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You are here:Home » Antibodies » Antibodies-Chromatin Proteins » Anti -SET 7/9

Anti -SET 7/9

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IC FC IF
SET 7/9 is a histone methyltransferase (HMTase) that transfers methyl group to Lys4 of histone H3, in complex with Sadenosyl-L-methionine (AdoMet). The methylation of lysine residues of histones plays a critical role in the regulation of chromatin structure and gene expression. Acetylation, phosphorylation and methylation of the amino-terminal tails of histone are thought to be involved in the regulation of chromatin structure and function. The enzymes identified in the methylation of specific lysine residue on histones belong to the SET family with just one exception. SET7/9, unlike most other SET proteins, is exclusively a monomethylase.
Catalog #S1012-23
ApplicationsSuitable for use in ELISA, Western Blot, Flow Cytometry and Immunofluorescence/Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:2000; Recombinant SET7/9 was resolved by electrophoresis, transferred to PVDF membrane and probed with S1012-23. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a ECL detection system.
Flow Cytometry: 2-5ug/10e6 cells
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2b,k
Clone No4k15
HostMouse
SourceHuman
ConcentrationAs Reported
FormSupplied as a liquid in PBS, pH 7.4 and 0.02% sodium azide, 10% glycerol.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein corresponding to aa1-366 from human SET 7/9 (NP_085151), purified from E. coli.
SpecificityRecognizes human SET 7/9.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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