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Anti -SFRP3 (Secreted Frizzled Related Protein 3, FRZB, Fritz, Frzb1)
Anti -SFRP3 (Secreted Frizzled Related Protein 3, FRZB, Fritz, Frzb1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B |
|
| sFRP-3, also known as FRZB, Fritz, Frzb1 and FRP-3, is expressed in the developing bone and cartilage as well as several adult tissues. It functions as an antagonist of Wnt | | | signaling. | | | Catalog # | S1012-88A | | Applications | Suitable for use in Western Blot and Direct ELISA. Other applications not tested. | | Recommended Dilution | Western Blot: 1-2ug/ml Detects human and mouse sFRRP-3. Using a colorimetric detection system, the detection limit for rhsFRP-3 is ~5ng/lane under non-reducing conditions. | | Direct ELISA: 0.5-1.0ug/ml Detects human and mouse sFRP-3. The detection limit for rhsFRP-3 is ~10ng/well. | | Optimal dilutions to be determined by the researcher. | | Hybridoma | Fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, NS0-derived, recombinant human secreted Frizzled Related Protein-3 (rhsFRP-3). | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 9j151 | | Host | Mouse | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in PBS, pH 7.2, 5% trehalose,40% glycerol | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | NS0-derived rhsFRP-3 | | Specificity | Recognizes human sFRP-3. Shows 100% crossreactivity with rmsFRP-3 and no crossreactivity with rhsFRP-1, rmsFRP-2 or rhsFRP-4. Species Crossreactivity: Mouse. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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