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You are here:Home » Antibodies » Abs to Wnt Proteins » Anti -SFRP3 (Secreted Frizzled Related Protein 3, FRZB, Fritz, Frzb1) (Biotin)

Anti -SFRP3 (Secreted Frizzled Related Protein 3, FRZB, Fritz, Frzb1) (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified B
Catalog #S1012-88B
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.1-0.2ug/ml. Used with the appropriate secondary reagents to detect human and mouse sFRP-3. The detection limit for rhsFRP-3 and rmsFRP-3 is 5ng/lane and 50ng/lane under non-reducing and reducing conditions, respectively.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, Tris-buffered saline containing 0.1% BSA. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.05mg/ml
FormSupplied as a lyophilized powder from PBS, pH 7.2 and BSA. Conjugated to Biotin. Reconstitute with 1ml sterile 40-50% glycerol, Tris-buffered saline, pH 7.3, containing 0.1% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenNS0-derived rhsFRP-3
SpecificityRecognizes human sFRP-3. Species Crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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