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Anti -SFRP3 (Secreted Frizzled Related Protein 3, FRZB, Fritz, Frzb1) (Biotin)
Anti -SFRP3 (Secreted Frizzled Related Protein 3, FRZB, Fritz, Frzb1) (Biotin)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
B IH IC |
|
| SFRP3, also known as FRZB, Fritz, Frzb1 and FRP-3, is expressed in the developing bone and cartilage as well as several adult tissues. It functions as an antagonist of Wnt | | | signaling. | | | Catalog # | S1012-88D | | Applications | Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 0.1-0.2ug/ml; The detection limit is ~5ng/lane under non-reducing and reducing conditions. In this format, shows ~60% crossreactivity with recombinant human SFRP3. | | Immunohistochemistry: 2-5ug/ml; Detects SFRP3 in cells and tissues. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile TBS, pH 7.3, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Goat | | Source | Mouse | | Concentration | ~0.05mg/ml | | Form | Supplied as a lyophilized powder from PBS, BSA. Labeled with Biotin. Reconstitute with 1ml sterile TBS, pH 7.3, 0.1% BSA. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Recombinant corresponding to mouse SFRP3 expressed in NS0 cells. | | Specificity | Recognizes mouse SFRP3. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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