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You are here:Home » Antibodies » Abs to Wnt Proteins » Anti -SFRP4 (Secreted Frizzled Related Protein 4, DDC-4, FrpAP, frpHE, FrzB-2)

Anti -SFRP4 (Secreted Frizzled Related Protein 4, DDC-4, FrpAP, frpHE, FrzB-2)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
sFRP4 belongs to the sFRP family whose members contain a cysteine-rich domain homologous to the Wnt-binding domain of the Frizzled proteins. SFRP4 is a circulating protein that can function as a phosphatonin as well as an antagonist of Wnt signaling.
Catalog #S1012-89
ApplicationsSuitable for use in Direct ELISA and Western Blot. Other applications not tested.
Recommended DilutionDirect ELISA: 0.5-1.0ug/ml. Detection limit for rhsFRP-4 is ~2ng/well.
Western Blot: 0.1-0.2ug/ml. Detection limit for rhsFRP-4 is ~20ng/lane under non-reducing and reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 24 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 5% trehalose, 40% glycerol.
PurityPurified by immunoaffinity chromatography. Endotoxin: < 0.1 EU per 1ug antibody (LAL method).
ImmunogenNS0-derived rhsFRP-4
SpecificityRecognizes human sFRP-4. Shows < 5% crossreactivity with rhsFRP-3, rhsFRP-1 and rmsFRP-2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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