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You are here:Home » Antibodies » Abs to Sonic Hedgehog (SHH) » Anti -SHH (Sonic HedgeHog, Sonic Hedgehog Protein, SHH, HHG-1)

Anti -SHH (Sonic HedgeHog, Sonic Hedgehog Protein, SHH, HHG-1)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified E B
Sonic Hedgehog Protein (SHH, VHH-1) belongs to hedgehog protein family which includes Indian Hh, and Desert Hh. Hh family is involved in the cell fate and patterning during embryonic development, homeostasis, and adult tissue renewal. Similar to other member in the family, SHH binds to the patched (PTC) cell surface receptor, releasing the signal transducer Smoothened (Smo) to transmit the Hh signal into the cell and activate transcription of the target gene. Precursor SHH is autocatlytically cleaved into two subunits, N-terminal and C-terminal products. Soluble N-terminal product is involved in the signaling activity, while C-product displays an autoproteolysis activity on the precursor and a cholesterol transferase activity on the N-terminal product. C-terminal product attaches a cholesterol moiety to the N-terminal product, preventing N-terminal product diffusion within the developing embryo. A defect in SHH has been linked in holoprosencephaly type 3 (HPE3), in which developing forebrain fails to separate into right and left hemispheres, and ocular coloboma.
Catalog #S1012-96G
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsWestern Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2A
Clone No11C50
HostRat
SourceMouse
ConcentrationNot determined
FormSupplied as a lyophilized powder from PBS, trehalose. Reconstitute with sterile PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenE. coli-derived recombinant mouse Sonic Hedgehog Cys25-Gly198 (Lys122Arg) (Accession # Q62226).
SpecificityRecognizes the N-terminal peptide of human (aa24-197) and mouse (aa25-198) Shh. Crossreactivity: N-terminal peptides of recombinant mouse Dhh (aa23-198) and rmIhh (aa66-240). No cross-reactivity with the C-terminal peptides from rmShh (aa199-437), rmDhh (aa199-396), or rmIhh (aa241-449).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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