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You are here:Home » Antibodies » Antibodies-Sonic Hedgehog (SHH) » Anti -Sonic Hedgehog (C-Product) (Sonic Hedgehog Homolog (Drosophila), HHG1, HHG-1, HLP3, HPE3, MCOPCB5, SMMCI, Sonic Hedgehog Protein, TPT, TPTPS)

Anti -Sonic Hedgehog (C-Product) (Sonic Hedgehog Homolog (Drosophila), HHG1, HHG-1, HLP3,
HPE3, MCOPCB5, SMMCI, Sonic Hedgehog Protein, TPT, TPTPS)


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Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH FC
Sonic Hedgehog Protein (SHH, VHH-1) belongs to hedgehog protein family which includes Indian Hh, and Desert Hh. Hh family is involved in the cell fate and patterning during embryonic development, homeostasis, and adult tissue renewal. Similar to other member in the family, SHH binds to the patched (PTC) cell surface receptor, releasing the signal transducer Smoothened (Smo) to transmit the Hh signal into the cell and activate transcription of the target gene. Precursor SHH is autocatlytically cleaved into two subunits, N-terminal and C-terminal products. Soluble N-terminal product is involved in the signaling activity, while C-product displays an autoproteolysis activity on the precursor and a cholesterol transferase activity on the N-terminal product. C-terminal product attaches a cholesterol moiety to the N-terminal product, preventing N-terminal product diffusion within the developing embryo. A defect in SHH has been linked in holoprosencephaly type 3 (HPE3), in which developing forebrain fails to separate into right and left hemispheres, and ocular coloboma.
Catalog #S1012-96L
ApplicationsSuitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:40
Western Blot: 1:2000
Immunohistochemistry (paraffin): 1:100
Immunoprecipitation: 1:70
Optimal dilutions to be determined by the researcher.
Positive ControlFetal liver membrane, human kidney tissue.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No9H273
ConcentrationNot determined
FormSupplied as a liquid in 50mM Tris-Glycine, pH7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
ImmunogenSynthetic peptide corresponding to residues within c-product subunit of human SHH. Cellular Localization: Secreted extracellular space.
SpecificityRecognizes both full length (50kD) and c-product subunit (27kD) of human SHH protein. Sonic hedgehog protein C-product is secreted and can be found in the extracellular space.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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