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SHP 1, Recombinant, Human, aa243-541 (SHPTP-1, SH2 containing protein tyrosine phosphatase 1, PTP1C, SH-PP1, HCP, SHP, PTPN6)
SHP 1, Recombinant, Human, aa243-541 (SHPTP-1, SH2 containing protein tyrosine phosphatase
1, PTP1C, SH-PP1, HCP, SHP, PTPN6)
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| The protein coding region of the catalytic domain of SHP-1 (amino acids 243-541). The catalytic domain of SHP-1 was overexpressed as insoluble protein aggregates (inclusion bodies). Recombinant SHP-1 protein was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a redox buffer. Additional amino acid(Met) is attached at N-terminus. Recombinant SHP-1 produced in E.Coli is a single,non-glycosylated polypeptide chain conjtaining 300 amino acids and having a molecular mass of 34.3kD. | | | Catalog # | S1013-25B | | Sequence | MGFWEEFES LQKQEVKNLH QRLEGQRPEN KGKNRYKNIL PFDHSRVILQ GRDSNIPGSDYINANYIKNQ LLGPDENAKT YIASQGCLEA TVNDFWQMAW QENSRVIVMT TREVEKGRNKCVPYWPEVGM QRAYGPYSVT NCGEHDTTEY KLRTLQVSPL DNGDLIREIW HYQYLSWPDHGVPSEPGGVL SFLDQINQRQ ESLPHAGPII VHCSAGIGRT GTIIVIDMLM ENISTKGLDCDIDIQKTIQM VRAQRSGMVQ TEAQYKFIYV AIAQFIETTK KKLEVLQSQK GQESEYGNITY | | Activity | ~5000 U/mg | | Unit Defenition | One unit will hydrolyze 1nanomole of p-nitrophenylphosphatate per minute at pH 7.5 at 37°C using 10mM of substrate. | | Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. | | Molecular Weight | 34.3kD | | Source | E. coli | | Purity | 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel. | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in 25mM Tris-HCl, pH 7.5, 2mM beta-mercaptoethanol, 1mM EDTA, 1mM DTT and 20% glycerol. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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