SHP 1, Recombinant, Human (SHPTP-1, SH2 containing protein tyrosine phosphatase 1, PTP1C,
SH-PP1, HCP, SHP, PTPN6)
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| Src-Homology 2 domain Phosphatase-1 (SHP-1), also known as Protein Tyrosine Phosphatase 1C (PTP1C), PTPN6, and Hematopoetic Cell Phosphatase (HCPH), is an enzyme that selectively dephosphorylates tyrosine residues in proteins. Spontaneous point mutations in the SHP-1 gene in mice produce the “motheaten” and motheaten viable” phenotypes that are severely autoimmune and immunodeficient.1 The enzyme is highly expressed in leukocyte cell types.2 SHP-1 has a regulatory region containing two Src homology 2 (SH2) domains that are critical for its binding to ITIM domains in inhibitory immunoreceptors.3 Deletion of the SH2 domains, as in this product, causes a marked increase in phosphatase activity.4 SHP-1 will dephosphorylate a wide variety of proteins, including the EGF receptor.5 A phosphopeptide containing the EGFR (Y992) sequence can be used to measure the activity of SHP-1 by detecting the release of phosphate. | | | Catalog # | S1013-25H | | Source | E. coli-expressed recombinant protein. | | Definition | Measured by the ability to dephosphorylate a tyrosine residue in a peptide containing the EGFR Y992 phosphorylation site in 10mM HEPES, pH 7.5, 0.1mM EDTA, 0.1mM EGTA, 5 mg/mL BSA. Assayed for 30 minutes at 37° C. | | Specific Activity | 20umoles/min/mg. | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: Predicted 46kD. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | 46kD | | Source | Recombinant Human | | Purity | 95%, as determined by SDS-PAGE and visualized by silver stain. | | Concentration | As reported | | Form | Supplied as a liquid in 50mM Tris, 0.15M sodium chloride, 50% glycerol, 1mM DTT, pH 7.5. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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