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You are here:Home » Molecular Biology » MB-Enzymes, Phosphatase » SHP 1, Recombinant, Human, GST-Tag (SHPTP-1, SH2 containing protein tyrosine phosphatase 1, PTP1C, SH-PP1, HCP, SHP, PTPN6)

SHP 1, Recombinant, Human, GST-Tag (SHPTP-1, SH2 containing protein tyrosine phosphatase
1, PTP1C, SH-PP1, HCP, SHP, PTPN6)

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Specifications

Protein tyrosine phosphatase, full length, (Gene bank accession number NM_080549) with N-terminal GST tag, expressed in an E. coli expression system.
Catalog #S1013-26H
Specific Activity1U/ug. One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.
Assay Buffer50mM HEPES, pH 7.4, 2mM EDTA, 3mM DTT, 100mM NaCl, 50mM pNPP.
ApplicationsUseful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityAliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
Molecular Weight95kD
SourceE. coli
Purity95% by SDS-PAGE
ConcentrationAs reported
FormSupplied as a liquid in 25mM Tris-HCl, pH 8.0, 75mM NaCl, 0.05% Tween-20, 50% glycerol, 2mM EDTA, 1mM DTT and 10mM glutathione.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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