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You are here:Home » Molecular Biology » MB-Enzymes, Phosphatase » SHP 1, Recombinant, Human, aa243-541 (SHPTP-1, SH2 Containing Protein Tyrosine Phosphatase 1, PTP1C, SH-PP1, HCP, SHP, PTPN6)

SHP 1, Recombinant, Human, aa243-541 (SHPTP-1, SH2 Containing Protein Tyrosine Phosphatase
1, PTP1C, SH-PP1, HCP, SHP, PTPN6)

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Specifications

The protein coding region of the catalytic domain of SHP-1 (amino acids 243-541) was cloned into an E. coli expression vector. The catalytic domain of SHP-1 was overexpressed as insoluble protein aggregates (inclusion bodies). Additional amino acid (Met) is attached at N-terminus.
Catalog #S1013-28
SequenceMGFWEEFESL/ QKQEVKNLHQ/ RLEGQRPENK/ GKNRYKNILP/ FDHSRVILQG/ RDSNIPGSDY/ INANYIKNQL/ LGPDENAKTY/ IASQGCLEAT/ VNDFWQMAWQ/ ENSRVIVMTT/ REVEKGRNKC/ VPYWPEVGMQ/ RAYGPYSVTN/ CGEHDTTEYK/ LRTLQVSPLD/ NGDLIREIWH/ YQYLSWPDHG/ VPSEPGGVLS/ FLDQINQRQE/ SLPHAGPIIV/ HCSAGIGRTG/ TIIVIDMLME/ NISTKGLDCD/ IDIQKTIQMV/ RAQRSGMVQT/ EAQYKFIYVA/ IAQFIETTKK/ KLEVLQSQKG/ QESEYGNITY
Specific Acitivity> 5000units/mg
Unit DefinitionOne unit will hydrolyze 1nanomole of p-nitrophenyl-phosphatate (pNPP) per minute at pH 7.4, 37°C using 10mM of substrate.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight34.3kD
SourceRecombinant SHP-1, human (produced in E. coli)
Purity95% by SDS PAGE; Purified by FPLC gel filtration chromatography, after refolding of the isolated inclusion bodies in a redox buffer. Endotoxin: 1EU/ug (LAL Method)
ConcentrationAs reported
FormSupplied as a liquid in 25mM Tris-HCl, pH 7.5, 2mM beta-mercaptoethanol, 1mM EDTA, 1mM DTT, 20% glycerol.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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