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You are here:Home » Molecular Biology » MB-Enzymes, Phosphatase » SHP 2, aa224-529, protein tyrosine phosphatase, catalytic domain, Recombinant, Human (SHPTP-2, PTP2C, SH-PTP2, PTP1D, SH-PTP3, Syp, PTPN11)

SHP 2, aa224-529, protein tyrosine phosphatase, catalytic domain, Recombinant, Human
(SHPTP-2, PTP2C, SH-PTP2, PTP1D, SH-PTP3, Syp, PTPN11)

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Specifications

SHP/SHPTP are tyrosine phosphatases which contain SH2 domains. SHP 1/SHPTP is expressed primarily in hematopoietic cells, and binds specific phosphotyrosine residues, often in ITIM motifs. SHP 1 is a down-regulator of receptor signaling and inhibits activation of macrophages, B-cells, T-cells, and mast cells. Motheaten mice harbor a mutation in SHP 1, which leads to progressive inflammation. SHP 2 has a broader expression pattern, and appears to promote cell proliferation and differentiation. Additionally, SHP 2 regulates cell spreading and migration in a manner similar to FAK, suggesting that the two act together to dynamically remodel focal adhesions.
Catalog #S1013-29B
Catalytic domain (aa224-529) of SHP 2 MW 33.5kD
Specific Activity: ~3u/ug.
Unit Definition: One unit will hydrolyze 1nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C
Assay buffer 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, 100 mM NaCl, 50mM pNPP.
Application Sutiable for use in Enzyme Kinetics, Regulation, to dephosphorylate target substrates and Screening inhibitors. Other applications not tested.
Recommended DilutionsOptimal dilutions to be determined by researcher.
Storage and StabilityAliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
Also AvailableS1013-29: SHP 2 (SHPTP-2) Pab Rb xMo
S1013-29A: SHP 2 Pab Rb xHu
S1013-29B: SHP 2 (224-529), protein tyrosine phosphatase, catalytic domain, Recombinant, Human (SHPTP-2, PTPN11)
S1013-29E: SHP 2, phosphorylated (Tyr542) Pab Rb xHu
S1013-29F: SHP 2, phosphorylated (Tyr580) Pab Rb xHu
SourceHuman, recombinant protein expressed E. coli
Purity> 95% by SDS-PAGE.
FormSupplied as a liquid in 25mM Tris-HCl, pH 8.0, 75mM sodium chloride, 0.05% Tween-20, 50% glycerol, 2mM EDTA, 3mM DTT.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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