Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Ubiquitin Ligases » Anti -Siah-1 (E3 ubiquitin-protein ligase SIAH1, Seven in absentia homolog 1, Siah-1a, SIAH1, HUMSIAH)

Anti -Siah-1 (E3 ubiquitin-protein ligase SIAH1, Seven in absentia homolog 1, Siah-1a,
SIAH1, HUMSIAH)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH IF
Siah-1 is a member of the seven in asentia homolog (SIAH) family. It is a E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins, including many proteins involved in transcription regulation, a cell surface receptor (DCC), cytoplasmic signal transduction molecules, an antiapoptotic protein (BAG1), a microtubule motor protein (KIF22), a protein involved in synaptic vesicle function in neurons (SYP) and a structural protein. Consequetly Siah-1 is involved in many cellular processes such as apoptosis, tumor suppression, cell cycle, axon gyidance, transcription regulation, spermatogenesis, and TNF-alpha signaling. It is predominantly a cytoplasmic protein but is also partialy a nuclear protein. It is widely expressed at low levels and is down-regulated in advanced hepatocellular carcinomas.
Catalog #S1013-31L7
ApplicationsSuitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunofluorescence: 10ug/ml
Immunohistochemistry: 3ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, ddH2O. Reconstituted product is stable for 12 months at -20°C. Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No9G442
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 100ul sterile ddH2O.
PurityPurified by affinity chromatography.
ImmunogenPartial recombinant human Shiah-1 (aa1-110) with a GST tag.
SpecificityRecognizes human Shiah-1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links