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You are here:Home » Antibodies » Abs to Lectins » Anti -Siglec H (Siglec h, Sialic Acid Binding Ig-like Lectin H, 6430529G09Rik, MGC106898)

Anti -Siglec H (Siglec h, Sialic Acid Binding Ig-like Lectin H, 6430529G09Rik, MGC106898)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified IH FC
Siglec-H is a cell-surface receptor molecule selectively expressed on murine natural interferon producing cells (IPC), also called mouse plasmacytoid dendritic cells (pDC). Siglec H is unique among Sialic acid-binding Ig-like lectins (Siglecs) proteins because it associates with the adaptor protein DAP12. DAP12 recognize certain viruses and CpG-DNA through TLR9, resulting in secretion of IFN-alpha, IL-12 and proinflammatory chemokines. Together these cytokines and chemokines recruite and activate NK cells and T cells as well as modulating the antigen presenting function of dendritic cells (DC). IPC themselves also function as antigen presenting cells that expand memory T cells and induce Th1 differentiation. Therefore IPC may provide a first line of host defense against viral infections by activating both innate and adaptive responses in vivo. This monoclonal antibody exclusively recognizes mouse Siglec-H in all lymphoid organs under both normal and inflammatory conditions. Siglec-H is normal present in the T cell zone of lymph nodes and spleen. Incubation of IPC with this antibody in vitro reduces secretion of IFN-alpha in response to CpG DNA without causing IPC depletion.
Catalog #S1013-63
ApplicationsSuitable for use in Flow Cytometry, Immunohistochemistry and Inhibition of IFN-alpha secretion by mouse interferon producing cells. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:50
Immunohistochemistry: Frozen
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No13A68
HostRat
SourceMouse
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, 0.1% BSA.
PurityPurified by Protein G affinity chromatography.
ImmunogenPurified bone-marrow derived mouse IPC.
SpecificityRecognizes mouse Siglec-H.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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