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Anti -Siglec-H (Siglech, Sialic Acid Binding Ig-like Lectin H, 6430529G09Rik, MGC106898) (Biotin)
Anti -Siglec-H (Siglech, Sialic Acid Binding Ig-like Lectin H, 6430529G09Rik, MGC106898)
(Biotin)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rat |
Affinity Purified |
IH FC |
|
| Siglec-H is a cell-surface receptor molecule selectively expressed on murine natural interferon producing cells (IPC), also called mouse plasmacytoid dendritic cells (pDC). Siglec H is unique among Sialic acid-binding Ig-like lectins (Siglecs) proteins because it associates with the adaptor protein DAP12. DAP12 recognize certain viruses and CpG-DNA through TLR9, resulting in secretion of IFN-alpha, IL-12 and proinflammatory chemokines. Together these cytokines and chemokines recruite and activate NK cells and T cells as well as modulating the antigen presenting function of dendritic cells (DC). IPC themselves also function as antigen presenting cells that expand memory T cells and induce Th1 differentiation. Therefore IPC may provide a first line of host defense against viral infections by activating both innate and adaptive responses in vivo. This monoclonal antibody exclusively recognizes mouse Siglec-H in all lymphoid organs under both normal and inflammatory conditions. Siglec-H is normal present in the T cell zone of lymph nodes and spleen. Incubation of IPC with this antibody in vitro reduces secretion of IFN-alpha in response to CpG DNA without causing IPC depletion. | | | Catalog # | S1013-63B | | Applications | Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Flow Cytometry: 1:50 | | Immunohistochemistry: Frozen | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2b | | Clone No | 13A68 | | Host | Rat | | Source | Mouse | | Concentration | ~0.1mg/ml | | Form | Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide. Labeled with Biotin. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Purified bone-marrow derived mouse IPC. | | Specificity | Recognizes mouse Siglec-H. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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