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Anti -Siglec-16 (Sialic Acid-binding Ig-like Lectin 16, SIGLEC16, Siglec-P16, SIGLECP16)
Anti -Siglec-16 (Sialic Acid-binding Ig-like Lectin 16, SIGLEC16, Siglec-P16, SIGLECP16)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
FC |
|
| Siglec-16 is a 58-60kD member of the CD33-related SIGLEC family of proteins. It is expressed on macrophages and microglia, and based on Siglec-11, likely binds to an a 2,8-linked sialic acid motif. Although Siglec-16 is assumed to have arisen from a Siglec-11 gene duplication and conversion, it is not an inhibitory receptor but an activating one, and possesses a transmembrane (TM) Lys that interacts with DAP12. Mature human Siglec-16 is a 465aa type I TM glycoprotein. It contains a 418aa extracellular region aa17-434 that shows one V-type aa 19-122 plus three C2-type aa147-424 Ig-like domains, and a short 26aa cytoplasmic tail. Notably, Siglec-16 exists as a pseudogene in ~50% of surveyed population. There is no Siglec-16 counterpart in either rodent or distant primate such as Rhesus monkey. The extracellular domain (ECD) of human Siglec-16 shares 94% aa identity with the ECD of human Siglec-11. | | | Catalog # | S1013-67T | | Applications | Suitable for use in Flow Cytometry. Other applications not tested. | | Recommended Dilution | Flow Cytometry: 2.5ug labels 10e6 cells | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2B | | Clone No | 12K176 | | Host | Mouse | | Source | Human | | Concentration | ~0.5mg/ml after reconstitution | | Form | Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml. | | Purity | Purified by Protein A/G affinity chromatography. | | Immunogen | Synthetic peptide PPARLSWTWGEQTVGPSQPSDPGVLQLPRV corresponding to aa369-398 from human Siglec-16. | | Specificity | Recognizes human Siglec-16. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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