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You are here:Home » Antibodies » Abs to Lectins » Anti -Siglec 16 (Sialic Acid-binding Ig-like Lectin 16, Siglec 16, Siglec P16, Siglec P16)

Anti -Siglec 16 (Sialic Acid-binding Ig-like Lectin 16, Siglec 16, Siglec P16, Siglec P16)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified FC
Siglec-16 is a 58-60kD member of the CD33-related SIGLEC family of proteins. It is expressed on macrophages and microglia, and based on Siglec-11, likely binds to an a 2,8-linked sialic acid motif. Although Siglec-16 is assumed to have arisen from a Siglec-11 gene duplication and conversion, it is not an inhibitory receptor but an activating one, and possesses a transmembrane (TM) Lys that interacts with DAP12. Mature human Siglec-16 is a 465aa type I TM glycoprotein. It contains a 418aa extracellular region aa17-434 that shows one V-type aa 19-122 plus three C2-type aa147-424 Ig-like domains, and a short 26aa cytoplasmic tail. Notably, Siglec-16 exists as a pseudogene in ~50% of surveyed population. There is no Siglec-16 counterpart in either rodent or distant primate such as Rhesus monkey. The extracellular domain (ECD) of human Siglec-16 shares 94% aa identity with the ECD of human Siglec-11.
Catalog #S1013-67T
ApplicationsSuitable for use in Flow Cytometry. Other applications not tested.
Recommended DilutionFlow Cytometry: 2.5ug labels 10e6 cells
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2B
Clone No12K176
HostMouse
SourceHuman
Concentration~0.5mg/ml after reconstitution
FormSupplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.
PurityPurified by Protein A/G affinity chromatography.
ImmunogenSynthetic peptide PPARLSWTWGEQTVGPSQPSDPGVLQLPRV corresponding to aa369-398 from human Siglec-16.
SpecificityRecognizes human Siglec-16.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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