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You are here:Home » Antibodies » Antibodies-Lectins » Anti -Specific Intracellular Adhesion Molecule Grabbing Nonintegrin R1 (SIGN-R1, CD209 antigen-like protein B)

Anti -Specific Intracellular Adhesion Molecule Grabbing Nonintegrin R1 (SIGN-R1, CD209
antigen-like protein B)


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Clone Host Grade Applications
Monoclonal Rat Supernatant IH FC
SIGN-R1 is a specific marker for the identification of macrophage subpopulations present in the marginal zone of spleen (the so-called marginal zone macrophages (MZM)), in the lymph node medulla, and in some strains, in the peritoneal cavity. Mouse SIGN-R1 is a C-type lectin, like DC-SIGN which is expressed on human dendritic cells (DCs). However, mouse SIGN-R1 itself is not expressed on DCs. SIGN-R1 exists in an aggregated form, resistant to dissociation into monomers upon boiling in SDS under reducing conditions. SIGN-R1 mediates the uptake of encapsulated organisms and may be an important mediator for the uptake of microbes in both spleen and lymph node, particularly through the recognition of microbial polysaccharides. Uptake of FITC-labeled dextran by macrophages can be blocked both in vivo and in vitro by the monoclonal antibody. Therefore, the monoclonal antibody can be used to study the uptake of polysaccharides by macrophages.
Catalog #S1013-69A
ApplicationsSuitable for use in Flow Cytometry, Functional Blocking Studies, and Immunohistochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:50
Immunohistochesmitry (frozen and paraffin): 1:50
For blocking studies in vitro, dilutions have to be made according to the amounts of SIGN-R1 to be inactivated. Optimal dilutions to be determined by the researcher.
Positive Control Peritoneal macrophages
Negative Control Alveolar macrophages
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No9G266
ConcentrationNot determined.
FormSupplied as a liquid in 0.02% sodium azide. Low Endotoxin.
ImmunogenMouse SIGN-R1.
SpecificityRecognizes murine SIGN-related 1 (SIGN-R1). Does not react with macrophages in other regions of the spleen, such as CD169+ marginal metallophils and F4/80+ red pulp macrophages. MZM of the spleen are involved in the clearance of polysaccharides.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesCD209 antigen-like protein B

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