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You are here:Home » Antibodies » Abs to Protein Kinase Substrates » Anti -SIRP, alpha1, aa487-503 (SIRPa, Signal Regulatory Protein, SHPS-1, BIT, p84, CD172a)

Anti -SIRP, alpha1, aa487-503 (SIRPa, Signal Regulatory Protein, SHPS-1, BIT, p84, CD172a)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Highly Purified B
Protein tyrosine phosphatases (PTPases) SHP-1 and SHP-2 are critical regulators in the intracellular signaling pathways that result in cell responses such as mitosis, differentiation, migration, survival, transformation or death. SHP-2 is a signal transducer for several receptor tyrosine kinases and cytokine receptors. A novel SHP-2 associated glycoprotein was recently cloned from human, rat, mouse and cattle by several labs and was designated SIRPa (1), SHPS-1 (2,3), MyD-1 (4), BIT (5,6) and p84 (7). SIRPa is a new gene family containing at least fifteen members. SIRPa is a substrate of many activated tyrosine kinases such as insulin receptor, EGFR, PDGFR and src, and a specific docking protein for SHP-2 (1,2,5,8). SIRPa has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion. It plays a general role in different physiological and pathological processes (1,2,5,8).
Catalog #S1013-87
ApplicationsSutiable for use in Western Blot. Other applications not tested.
Recommended DilutionsWestern Blot: 1:1000-1:2000 dilution. Detects bands at 75-110kD may be SHPTP 1 degradation products.
Optimal dilutions to be determined by researcher.
Positive Control Whole cell lysate from THP-1 cells.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, 0.02% sodium azide.
PurityPurified by DEAE chromatography.
ImmunogenSynthetic peptide of human SIRPa1 corresponding to aa487-503 (KPEPSFSEYASVQVPRK).
SpecificityRecognizes SIRPa1. The sequences of immunogenic peptide differ from those of mouse, rat and bovine by one amino acid.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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