Anti -SIRP, alpha1, aa487-503 (SIRPa, Signal Regulatory Protein, SHPS-1, BIT, p84, CD172a)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Highly Purified |
B |
|
| Protein tyrosine phosphatases (PTPases) SHP-1 and SHP-2 are critical regulators in the intracellular signaling pathways that result in cell responses such as mitosis, differentiation, migration, survival, transformation or death. SHP-2 is a signal transducer for several receptor tyrosine kinases and cytokine receptors. A novel SHP-2 associated glycoprotein was recently cloned from human, rat, mouse and cattle by several labs and was designated SIRPa (1), SHPS-1 (2,3), MyD-1 (4), BIT (5,6) and p84 (7). SIRPa is a new gene family containing at least fifteen members. SIRPa is a substrate of many activated tyrosine kinases such as insulin receptor, EGFR, PDGFR and src, and a specific docking protein for SHP-2 (1,2,5,8). SIRPa has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion. It plays a general role in different physiological and pathological processes (1,2,5,8). | | | Catalog # | S1013-87 | | Applications | Sutiable for use in Western Blot. Other applications not tested. | | Recommended Dilutions | Western Blot: 1:1000-1:2000 dilution. Detects bands at 75-110kD may be SHPTP 1 degradation products. | | Optimal dilutions to be determined by researcher. | | Positive Control | Whole cell lysate from THP-1 cells. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in PBS, 0.02% sodium azide. | | Purity | Purified by DEAE chromatography. | | Immunogen | Synthetic peptide of human SIRPa1 corresponding to aa487-503 (KPEPSFSEYASVQVPRK). | | Specificity | Recognizes SIRPa1. The sequences of immunogenic peptide differ from those of mouse, rat and bovine by one amino acid. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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