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You are here:Home » Antibodies » Antibodies-Protein Kinase Substrates » Anti -SIRP, alpha1 (SIRPa, Signal Regulatory Protein, SHPS-1, BIT, p84, CD172a)

Anti -SIRP, alpha1 (SIRPa, Signal Regulatory Protein, SHPS-1, BIT, p84, CD172a)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH FC
Catalog #S1013-87H
Recommended Secondary Reagents Goat anti-mouse IgG: HRP conjugate (rat absorbed), IgG Goat anti mouse IgG: RPE (rat adsorbed)
Fusion Partners Spleen cells from immunized BALB/c mice were fused with the NSO/U mouse myeloma cell line.
Recommended Negative Controls Mouse IgG1 Negative Control
ApplicationsFlow Cytometry: 1:10-1:50
Immunohistochemistry: Frozen, Paraffin
Western Blot
Optimal working dilutions to be determined by researcher.
Flow Cytometry Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.
Immunohistochemistry: Requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Clone TypeMonoclonal
Clone No3H1939
FormPurified IgG - liquid with 0.1% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenRat peritoneal macrophages.
SpecificityRecognizes rat CD172a, also known as Signal regulatory protein (SIRP). CD172a is expressed selectively by myeloid cells and by neurons. 3H1939 binds to a different epitope on SIRP than ED9. It does not block the interaction of CD172a-CD47.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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