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Anti -SIRP, alpha1 (SIRPa, Signal Regulatory Protein, SHPS-1, BIT, p84, CD172a) (Azide Free)
Anti -SIRP, alpha1 (SIRPa, Signal Regulatory Protein, SHPS-1, BIT, p84, CD172a) (Azide Free)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B IH FC |
|
| Catalog # | S1013-87J | | Recommended Secondary Reagents | Goat anti-mouse IgG: HRP conjugate (rat absorbed), IgG Goat anti mouse IgG: RPE (rat adsorbed) | | Fusion Partners | Spleen cells from immunized BALB/c mice were fused with the NSO/U mouse myeloma cell line. | | Recommended Negative Controls | Mouse IgG1 Negative Control | | Applications | Flow Cytometry: 1:10-1:50 | | Immunohistochemistry: Frozen, Paraffin. | | Western Blot | | Optimal working dilutions to be determined by researcher. | | Flow Cytometry | Use 10ul of the suggested working dilution to label 10e6 cells in 100ul. | | Immunohistochemistry: Requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2a | | Clone No | 3H1939 | | Host | Mouse | | Source | Rat | | Concentration | ~1mg/ml | | Form | Purified IgG - liquid. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Rat peritoneal macrophages. | | Specificity | Recognizes rat CD172a, also known as Signal regulatory protein (SIRP). CD172a is expressed selectively by myeloid cells and by neurons. 3H1939 binds to a different epitope on SIRP than ED9. It does not block the interaction of CD172a-CD47. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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