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You are here:Home » Antibodies » Abs to Protein Kinase Substrates » Anti -SIRP, alpha1(SIRPa, Signal Regulatory Protein, SHPS-1, BIT, p84, CD172a)

Anti -SIRP, alpha1(SIRPa, Signal Regulatory Protein, SHPS-1, BIT, p84, CD172a)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH FC
Human CD172a, also known as signal-regulatory protein alpha, a receptor-type transmembrane glycoprotein expressed on cells of myeloid origin, including granulocytes, dendritic cells (DCs),
macrophages, mast cells and haematopoietic stem cells. CD172a acts as a substrate for several activated tyrosine kinases, including EGFR, PDGFR, src and insulin receptor and is involved in the negative regulation of receptor tyrosine kinase-coupled signaling pathways. Ligand binding of CD172a to integrin-associated protein CD47, results in tyrosine kinase phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within the cytoplasmic region of CD172a, mediating the recruitment and activation of the tyrosine phosphatases SHP-1 and SHP-2. These then act as regulators of cellular function, through dephosphorylation of specific substrates. Ligation of CD172a with CD47 has been demonstrated in several regulatory processes, including the inhibition of host cell phagocytosis by macrophages and the bi-directional activation of T cells and DCs.
Catalog #S1013-87J1
ApplicationsSuitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:25-1:100. Use 10ul to label 1x10e6 cells in 100ul.
Immunohistochemistry (Frozen): 1:100
Optimal dilutions to be determined by the researcher.
HybridomaSpleen cells from immunized Balb/c mice were fused with cells of the X63-Ag8.653 myeloma cell line
Recommended Positive Control (Ihc)Human Tonsil
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No9A204
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH7.2, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenMonocyte-derived dendritic cells.
SpecificityRecognizes human SIRP, alpha1 (CD172a).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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