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You are here:Home » Antibodies » Abs to Protein Kinase Substrates » Anti -SIRP, alpha1 (SIRPa, Signal Regulatory Protein, SHPS-1, BIT, p84, CD172a)

Anti -SIRP, alpha1 (SIRPa, Signal Regulatory Protein, SHPS-1, BIT, p84, CD172a)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified FC
Catalog #S1013-87Q
Applications
Suitable for use inFlow Cytometry and Immunohistochemistry (frozen and paraffin). Other applications not tested.
Recommended DilutionFlow Cytometry: 1:100-1:200 Use 10ul to label 10e6 cells in 100ul
Immunohistochemistry: Requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Optimal dilution determined by the researcher
Recommended Secondary ReagentsIgG Goat anti mouse IgG:FITC (rat adsorbed)
IgG Goat anti mouse IgG:RPE (rat adsorbed)
ImmunohistochemistryRecommended Secondary Reagents:
Goat anti mouse IgG:HRP (rat adsorbed)
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No6A459
HostMouse
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenRat peritoneal macrophages
SpecificityRecognizes rat CD172a, also known as Signal regulatory protein (SIRP). CD172a is expressed selectively by myeloid cells and by neurons. Binds to a different epitope on SIRP than ED9. Does not block the interaction of CD172a-CD47.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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