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Anti -SIRP, alpha1 (SIRPa, Signal Regulatory Protein, SHPS-1, BIT, p84, CD172a)
Anti -SIRP, alpha1 (SIRPa, Signal Regulatory Protein, SHPS-1, BIT, p84, CD172a)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
FC |
|
| Catalog # | S1013-87Q | | Applications | | | Suitable for use inFlow Cytometry and Immunohistochemistry (frozen and paraffin). Other applications not tested. | | Recommended Dilution | Flow Cytometry: 1:100-1:200 Use 10ul to label 10e6 cells in 100ul | | Immunohistochemistry: Requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase. | | Optimal dilution determined by the researcher | | Recommended Secondary Reagents | IgG Goat anti mouse IgG:FITC (rat adsorbed) | | IgG Goat anti mouse IgG:RPE (rat adsorbed) | | Immunohistochemistry | Recommended Secondary Reagents: | | Goat anti mouse IgG:HRP (rat adsorbed) | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2a | | Clone No | 6A459 | | Host | Mouse | | Source | Rat | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Rat peritoneal macrophages | | Specificity | Recognizes rat CD172a, also known as Signal regulatory protein (SIRP). CD172a is expressed selectively by myeloid cells and by neurons. Binds to a different epitope on SIRP than ED9. Does not block the interaction of CD172a-CD47. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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