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You are here:Home » Molecular Biology » MB-Sirtuins » SIRT 2, Recombinant, Human (Sirtuin 2, Silent Mating Type Information Regulation 2, S. cerevisiae Homolog 2)

SIRT 2, Recombinant, Human (Sirtuin 2, Silent Mating Type Information Regulation 2, S.
cerevisiae Homolog 2)

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Specifications

Human Sirtuin 2 (GenBank Accession No. NM_030593), amino acids 50-356 with C-terminal His tag, expressed in E.coli expression system.
Catalog #S1013-88C
Specific Activity1.4 U/ug. One U =1 pmol/min
Assay Condition25mM Tris/Cl, pH 8.0, 137mM NaCl, 2.7mM KCl, 1mM MgCl2 and 0.1mg/ml BSA, 20uM BPS HDAC substrate, 500uM NAD+ and 100ng/ul Sirtuin 2.
Incubation Condition30 min at 37ºC.
ApplicationsUseful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityAliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
Molecular Weight35.5kD
SourceE. coli
Purity90%
ConcentrationAs reported
FormSupplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM NaCl, 0.05% Tween-20, 50% glycerol and 3mM DTT.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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