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You are here:Home » Molecular Biology » MB-Sirtuins » SIRT 3, Recombinant, Human, GST-Tag (Sirtuin 3, Sir2-related Protein Type 3, Sir2-like 3)

SIRT 3, Recombinant, Human, GST-Tag (Sirtuin 3, Sir2-related Protein Type 3, Sir2-like 3)

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Specifications

Human Sirtuin 3 (GenBank Accession No. NM_012239), amino acids 102- 399 with N-terminal GST tag, expressed in E. coli expression system.
Catalog #S1013-88F2
Specific Activity6 U/ug. One U =1 pmol/min
Assay Condition25mM Tris/Cl, pH 8.0, 137mM NaCl, 2.7mM KCl, 1mM MgCl2 and 0.1mg/ml BSA, 30uM BPS HDAC substrate, 200uM NAD+ and 20ng/ul Sirtuin 3.
Incubation Condition 30 min at 30ºC
ApplicationUseful for the study of enzyme kinetics, screening inhibitors and selectivity profiling.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityAliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
Molecular Weight59kD
SourceE. coli
Purity90% by SDS-PAGE
ConcentrationAs reported
FormSupplied as a liquid in 50mM Tris-HCl, pH 8.0, 138mM NaCl, 20mM glutathione and 10% glycerol.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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