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SIRT 3, Recombinant, Human (Sirtuin 3, Sir2-related Protein Type 3, Sir2-like 3)
SIRT 3, Recombinant, Human (Sirtuin 3, Sir2-related Protein Type 3, Sir2-like 3)
Pricing
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| Human Sirtuin 3 (GenBank Accession No. NM_012239), amino acids 102- 399 with N-terminal GST tag, expressed in E. coli expression system. | | | Catalog # | S1013-88F2 | | Specific Activity | 6 U/ug. One U =1 pmol/min | | Assay Condition | 25mM Tris/Cl, pH 8.0, 137mM NaCl, 2.7mM KCl, 1mM MgCl2 and 0.1mg/ml BSA, 30uM BPS HDAC substrate, 200uM NAD+ and 20ng/ul Sirtuin 3. | | Incubation Condition | 30 min at 30ºC | | Application | Useful for the study of enzyme kinetics, screening inhibitors and selectivity profiling. | | Recommended Dilution | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months. | | Molecular Weight | 59kD | | Source | E. coli | | Purity | 90% by SDS-PAGE | | Concentration | As reported | | Form | Supplied as a liquid in 50mM Tris-HCl, pH 8.0, 138mM NaCl, 20mM glutathione and 10% glycerol. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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