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Anti -SIRT 5 (Sirtuin 5, Sirtuin-5, NAD-dependent Deacetylase Sirtuin-5, SIR2-like Protein 5 SIR2L5) (Biotin)
Anti -SIRT 5 (Sirtuin 5, Sirtuin-5, NAD-dependent Deacetylase Sirtuin-5, SIR2-like Protein
5 SIR2L5) (Biotin)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Sheep |
Affinity Purified |
B |
|
| Sirtuin 5 (SIRT5; SIR2-like protein 5) is a 28-35kD class III member of the sirtuin protein family. It is found in the mitochondrial matrix and is involved in ammonia detoxification. In particular, it deacetylates carbamoyl phosphate synthetase 1/CPS1, thus activating it and allowing for ammonia condensation with bicarbonate and ATP. This is a crucial step in urea formation during prolonged fasting or the use of a high-protein diet. Human SIRT5 is 310aa in length. It possesses a sertuin-type deacetylase domain (aa41-309) that contains an NAD binding motif (Thr276:Glu277:Pro292). There are three potential isoform variants that show aa substitutions at the C-terminus. One contains a 14aa substitution for aa286-310, a second shows a 15aa substitution for aa287-310, and the third shows a 17aa substitution for aa207-310. Full-length human and mouse SIRT5 share 86% aa identity. | | | Catalog # | S1013-88J6 | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilutions | Western Blot: 0.1-0.2ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile TBS, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Sheep | | Source | Human | | Concentration | Not determined | | Form | Supplied as a lyophilized powder from PBS, 50ug/1ug BSA. Labeled with Biotin. Reconstitute with sterile TBS, pH 7.3, 20mM Trizma, 150mM sodium chloride, 0.1% BSA. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Purified, Sf 21-derived, recombinant human SIRT5 isoform 1 (rhSIRT5; aa33-310; Accession # Q9NXA8). | | Specificity | Recognizes human SIRT5. Species sequence homology: mouse (86%). | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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