Anti -SKI (Sarcoma Viral Oncogene Homolog, Avian, c-SKI, SKV, v-Ski)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B |
|
| Three Smad transcriptional co-repressors, namely Ski (Sloan-Kettering Institute proto-oncogene), SnoN (Ski-related novel gene, non Alu-containing), and TGIF (TG-interacting factor), have been identified (25,27–29). They exert transcriptional repression by numerous mechanisms (30–33). Clearly, Smad transcriptional co-repressors are unique regulatory components within the nuclei during the final stage of TGF-1 signaling; hence, their abundance and activity allow the cell to make the decision whether to proceed with the transcription of TGF-1–responsive genes. However, despite the obvious importance of Smad transcriptional co-repressors in controlling TGF-1 signaling, the regulation and relative significance of their expression in fibrotic kidney are completely unknown. | | | Catalog # | S1013-89 | | SKI is an 80-90 kD member of the SKI family of oncoproteins. SKI is best known for inhibiting TGF- signaling through its association with the SMAD proteins. Human SKI is 728 aa long and contains an N-terminal Cys-rich region (aa 94-192) and a C-terminal coiled-coil oligomerization domain (aa 536-710). Over the region used as immunogen, human and mouse SKI protein sequences are 96% identical. | | The Ski and Sno proteins are components of a macromolecular complex containing the co-repressor N-CoR/SMRT, mSin3 and histone deacetylase. The complex mediates repression by a number of nuclear hormone receptors and Mad, and MeCP2. In addition, Ski and Sno can render cells resistant to TGF-beta by binding to Smads. | | Ski (v-ski) was discovered as a viral oncogene from the genome of avian Sloan-Kettering retrovirus. Overexpression of both v-ski and cellular homolog c-ski in avian fibroblasts leads to anchorage-independent growth and morphological transformation of chicken embryo fibroblasts. Overexpression of c-ski has also been observed in several types of human cancer cells. New results show that Ski associates with Smads2, 3 and 4 proteins in response to TGF- stimulation. Interactions between Ski and Smad2, 3 and 4 blocks Smads-mediated transcriptional activation as well as TGF- -induced growth suppression of epithelial cells, suggesting oncogenic effects of Ski derive in part through its ability to antagonize the growth-inhibitory signals initiated by members of the TGF- family of ligands. Ski is also required for transcriptional repression mediated by Mad involved in negative regulation of cellular proliferation and plays a role in muscle differentiation.Ski (v-ski) was discovered as a viral oncogene from the genome of avian Sloan-Kettering retrovirus. Overexpression of both v-ski and cellular homolog c-ski in avian fibroblasts leads to anchorage-independent growth and morphological transformation of chicken embryo fibroblasts. Overexpression of c-ski has also been observed in several types of human cancer cells. New results show that Ski associates with Smads2, 3 and 4 proteins in response to TGF- stimulation. Interactions between Ski and Smad2, 3 and 4 blocks Smads-mediated transcriptional activation as well as TGF- -induced growth suppression of epithelial cells, suggesting oncogenic effects of Ski derive in part through its ability to antagonize the growth-inhibitory signals initiated by members of the TGF- family of ligands. Ski is also required for transcriptional repression mediated by Mad involved in negative regulation of cellular proliferation and plays a role in muscle differentiation. | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 2-4ug/ml detects Ski in HeLa nuclear extract. | | HeLa nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Ski (2ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. | | Optimal dilutions to be determined by the researcher. | | Positive Antigen Control | H1838 | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~0.8mg/ml | | Form | Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | His-tagged fusion protein corresponding to residues 553-728 of human Ski. | | Specificity | Recognizes Ski, Mr ~95kD, as well as phosphorylated forms, Mr 105 and 115kD. An additional unidentified band is observed in some lysates, Mr 68kD. Species Crossreactivity: Human and mouse. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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